Genetic variability of carbapenemase KPC-producing Klebsiella pneumoniae isolated at different states in Venezuela

Nirvia Margot Cuaical-Ramos; Marynes Montiel; Daniel Marcano Zamora

doi:10.1016/j.eimc.2017.12.004

Genetic variability of carbapenemase KPC-producing Klebsiella pneumoniae isolated at different states in Venezuela

Enferm Infecc Microbiol Clin (Engl Ed). 2019 Feb;37(2):76-81. doi: 10.1016/j.eimc.2017.12.004. Epub 2018 Feb 13.

[Article in English, Spanish]

Authors

Nirvia Margot Cuaical-Ramos¹, Marynes Montiel², Daniel Marcano Zamora³

Affiliations

¹ Instituto Nacional de Higiene Rafael Rangel, Departamento de Bacteriología, Ciudad Universitaria (UCV), Los Chaguaramos, Caracas, República Bolivariana de Venezuela. Electronic address: ramosnirvia@hotmail.com.
² Unidad de Investigación en Microbiología Ambiental, Facultad Experimental de Ciencias, Universidad del Zulia, Maracaibo, República Bolivariana de Venezuela; Escuela Superior Politécnica del Litoral, ESPOL, Facultad de Ciencias de la Vida, Campus Gustavo Galindo, Guayaquil, Ecuador.
³ Instituto Nacional de Higiene Rafael Rangel, Departamento de Bacteriología, Ciudad Universitaria (UCV), Los Chaguaramos, Caracas, República Bolivariana de Venezuela.

PMID: 29422290
DOI: 10.1016/j.eimc.2017.12.004

Abstract

Introduction: In Venezuela, there have been some reports of carbapenemase KPC-producing Klebsiella pneumoniae. Nevertheless, since the first report in 2008, only a few studies have been done on their molecular epidemiology in this country.

Methods: The aims of this study were to detect extended-spectrum betalactamase (ESBL)-producing (blaTEM and blaCTM-M-1) and to determine the genetic relationship between 30 isolates of carbapenemase KPC-producing K. pneumoniae taken from patients at eleven health centers in different states of Venezuela from January 2008 to December 2012, using pulsed-field gel electrophoresis (PFGE).

Results: All isolates were identified as K. pneumoniae subsp. pneumoniae. Isolates showed the highest resistance to the ertapenem, 97%. The KPC gene was detected in all studied strains. Seventy three percent showed ESBL, having the bla_TEM in 68% and bla_TEM, _CTX-M-1 in 27% of the strains. Eleven groups were found using the field-pulsed gel electrophoresis.

Conclusion: High genetic diversity was found during 2008-2012 in K. pneumoniae isolated at different states in Venezuela, some of them circulating at eleven health centers. Results showed the importance of performing epidemiologic studies and the need to develop some activities to control this type of microorganisms.

Keywords: Genetic variability; Klebsiella pneumoniae; Variabilidad genética; Venezuela.

MeSH terms

Bacterial Proteins / analysis*
Bacterial Proteins / genetics
Drug Resistance, Multiple, Bacterial / genetics
Electrophoresis, Gel, Pulsed-Field
Genes, Bacterial*
Genetic Variation
Genotype
Geography, Medical
Humans
Klebsiella Infections / epidemiology
Klebsiella Infections / microbiology*
Klebsiella pneumoniae / enzymology
Klebsiella pneumoniae / genetics*
Klebsiella pneumoniae / isolation & purification
Microbial Sensitivity Tests
Molecular Epidemiology
Venezuela
beta-Lactam Resistance / genetics*
beta-Lactamases / analysis*
beta-Lactamases / genetics

Substances

Bacterial Proteins
beta-lactamase TEM-3
beta-Lactamases
carbapenemase